Ikholamu yeGhost-Sniper yeChromasir HPLC UPLC ikholamu isuse iincopho zesiporho

Ikholamu yesiporho-Sniper yeChromasir HPLC UPLC ikholamu susa iincopho zesiporho Umfanekiso ofakiweyo

inkcazelo emfutshane:

Ikholamu ye-Ghost-Sniper sisixhobo esinamandla sokuphelisa iincopho zesiporho eziveliswe ngexesha lenkqubo yokwahlulwa kwechromatographic, ngakumbi kwimo yegradient. Incopho yesiporho iya kubangela iingxaki zobungakanani ukuba iincopho zesiporho zidlula incopho zomdla. Ngekholamu yeChromasir ghost-sniper, yonke imiceli mngeni yokuphakama kwesiporho inokusonjululwa kwaye iindleko zokusebenzisa umfuniselo zinokuhla kakhulu.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ikholamu ye-Ghost-sniper yenziwe ngokukodwa ukuphelisa iincopho zesiporho. Iincopho zesiporho ziyimvelaphi engaziwayo kwichromatogram, edla ngokubonwa ngexesha lenkqubo yokwahlulwa kwechromatographic kwi-gradient elution okanye ukusebenza kwexesha elide. Ukwenzeka kwencopho yeziporho kunempembelelo enzulu kuvavanyo lwabahlalutyi. Ukholo lwe-Ghost-sniper lwenzeka ukusombulula ezo ngxaki kwaye yenze inkqubo yovavanyo ibe lula kwaye isebenze kakuhle. Le kholamu ingasetyenziswa kwimeko egqithisileyo kwaye ibonisa umphumo omkhulu wokubamba. Ngokuqinisekileyo yindlela elungileyo yokuphelisa uphazamiseko olusuka kwincopho yesiporho kwindlela yokuqinisekisa kunye nokulandela uhlalutyo lwento.

Iiparamitha

Icandelo elinguNomb.	Ubungakanani	Umthamo	Isicelo
MC5046091P	50×4.6mm	malunga ne-800ul	HPLC
MC3546092P	35×4.6mm	malunga ne580ul	HPLC
MC5021093P	50 × 2.1mm	malunga ne-170ul	I-UPLC
MC3040096P	30 × 4.0mm	malunga ne-380ul	HPLC Umthamo wekholamu ephantsi

Ukuhlohla

Isicelo kunye neZiphumo

Ukulumkela

1. Ukuba uhlalutyo lwebhetshi lulungiselelwe kwinkqubo ye-HPLC, kucetyiswa ukuba kwandiswe ixesha elongezelelweyo lokulinganisela malunga ne-5 min - i-10 min kwiimeko zakho ze-chromatographic, ngenxa yempembelelo yomthamo wekholamu ye-ghost-sniper.
2. Kwiikholomu ezintsha, zihlambulule nge-100% i-acetonitrile kwinqanaba lokuhamba kwe-0.5ml / min kwiiyure ezi-4 ngaphambi kokusetyenziswa.
3. Ii-reagents ze-Ion-pair kwisigaba esihambayo, zinokufunxwa yikholomu yesiporho, enokuthi ibe nefuthe kwixesha lokugcinwa kunye nokuma kwencopho yethagethi yakho. Nceda usebenzise ngononophelo phantsi kwesigaba esiphathwayo esinjalo.
4. Ubomi bekholomu buxhomekeke kwiimeko zokuhlalutya, njengesigaba seselula. ukucoceka kwe-solvent, kunye nokungcoliswa kwesixhobo. Nceda ubuyisele ikholamu yesiporho-sniper rhoqo ukuze uqinisekise ukusebenza.
5. Ukutshintsha ikholam ye-ghost-sniper kucetyiswa ukuba i-capturing effect iba mbi kakhulu okanye iimfuno azinakwaneliswa.
6. Njengenxalenye yokucoca kwi-chromatograph ye-liquid, ikholamu ye-ghost-sniper inokucoca amaqhekeza aqinile kwaye isuse izinto ezingcolileyo ze-organic phambi kwe-injector. Ikholamu yesiporho-sniper ikwabonelela ngezixhobo kunye nekholamu yechromatographic ngokhuseleko olungcono, kwaye ifezekisa ichromatogram.
7. Ukuba i-mobile phase ine-buffer salt, ngaphambi nangemva kokusetyenziswa, thumela ukugungxula nge-10% yesisombululo se-organic phase (10% methanol okanye i-acetonitrile), ukuphepha i-buffer salt out and block the column.
8. Nceda uqaphele ukuba ayizizo zonke iincopho zesiporho ezinokubanjwa yiKholam ye-Ghost-Sniper.
9. Ukuba ikholamu ishiya ingasetyenziswanga ixesha elide, kucetyiswa ukuba uyigcine kwisisombululo se-organic aqueous (70% methanol okanye i-acetonitrile). Kwaye nceda uyiguqule nge-100% i-acetonitrile kwisantya sokuhamba kwe-0.5 ml / min ngeyure eli-1 ngaphambi kokusebenzisa.