I-Chromatography yindlela eyimfuneko kuphando lwezenzululwazi lwangoku, kodwa ukuvela kweiincopho zesiporhokwiichromatograms kunokubangela imingeni ebalulekileyo kubahlalutyi. Ezi zincopho zingalindelekanga, zihlala zivela ngexesha lokwahlulwa kwechromatographic, ngakumbi kwimowudi yegradient, iphazamisa uhlalutyo lobungakanani kunye nokuchitha ixesha elixabisekileyo. Ukuqonda oonobangela bencopho yesiporho kunye nokusebenzisa izisombululo ezintsha ezifana neChromasir'sIKholam yesiporho-Sniperinokuguqula iinkqubo zakho zohlalutyo.
Ziziphi iiNqaku zeGhost kwaye kutheni zibalulekile?
Incopho yesiporho yimiqondiso engachazwanga kwichromatogram ephazamisana nokucaca kweziphumo zohlalutyo. Ngelixa zinokubonakala zincinci, impembelelo yazo inokubaluleka:
1. Imingeni yobungakanani
Xa iincopho zesiporho zidibana nencopho yomdla, zenza nzima ubungakanani obuchanekileyo babahlalutyi. Oku kunokukhokelela ekutolikweni kwedatha ephosakeleyo kunye neziphumo ezingathembekanga.
2. Ukucombulula iingxaki ezidla ixesha
Ukuchonga imvelaphi yeencopho zeziporho kudla ngokufuna uphando olude, luphambukisa ujoliso lwabahlalutyi kwimisebenzi ebalulekileyo. Ixesha elichithwe ekusombululeni le miba lingaphucula imveliso kunye neziphumo zophando.
Zivela phi iiNcopho zeGhost?
Ukuphelisa ngempumelelo iincopho zesiporho, kubalulekile ukuqonda imvelaphi yazo. Ezi ncopho zingalindelekanga zidla ngokuvela kwizinto ezingcolisayo:
1.Amacandelo eNkqubo:Iintsalela kwinkqubo yechromatographic zinokufaka isandla kwiincopho zesiporho.
2.Imihlathi:Ukungcola kwimpahla yokupakisha okanye ukuguga kokusetyenziswa kunokubangela ungcoliseko.
3.Iisampulu:Iisampulu ezingcolisekileyo zazisa iikhompawundi ezingalindelekanga kwichromatogram.
4.Isigaba esiphathwayo:Ukungcola okuvela kwizinyibilikisi, iityuwa ze-buffer, okanye izigaba ezinamanzi/eziphilayo zihlala zinegalelo kwiincopho zeziporho.
5.Izikhongozeli:Iibhotile zokulungiselela iisampulu kunye nezinye izikhongozeli zinokuzisa ungcoliseko olushiyekileyo.
Isisombululo seNguquko: IKholamu yeGhost-Sniper
I-Chromasir'sIKholam yesiporho-Snipersisisombululo esitshintsha umdlalo esenzelwe ukulwa iincopho zesiporho. Le kholamu yesizukulwana sesibini sibonisa isakhiwo esiphuculweyo kunye nezixhobo zokupakisha eziphambili, ezivumela ukuba zibambe ngokufanelekileyo iincopho zesiporho naphantsi kweemeko ezinzima. Ukusebenza kwayo kuyenza ibe sisixhobo esibalulekileyo sokuqinisekisa indlela kunye nohlalutyo lomkhondo.
Iilebhu ezisebenzisa iKholamu ye-Ghost-Sniper ichaze uphuculo olubonakalayo kumgangatho wechromatogram, amaxesha ancitshisiweyo okusombulula ingxaki, kunye nokuphucula imveliso iyonke.
Ungazinyusela njani iiNzuzo zeKholamu ye-Ghost-Sniper
Ukuze usebenze ngokugqibeleleyo, landela la manyathelo okhuseleko kunye nezenzo ezilungileyo:
1.Ulungelelwaniso Lwexesha Lomncono:
Yongeza imizuzu emi-5 ukuya kwe-10 yexesha lokulinganisa kwiinkqubo ze-HPLC ukulungiselela umthamo wekholomu.
2.Ukuseta okokuqala:
Gcoba iikholomu ezintsha nge-100% i-acetonitrile kwisantya sokuhamba kwe-0.5 mL / min kwiiyure ze-4 ngaphambi kokusetyenziswa ukuqinisekisa ukusebenza okucocekileyo nokusebenzayo.
3.Phatha iiReagents ze-Ion-Pair ngononophelo:
I-Ion-pair reagents kwisigaba esihambayo sinokutshintsha amaxesha okugcina kunye neemilo eziphezulu. Sebenzisa ngononophelo xa kukho ii-reagents ezinjalo.
4.tshintsha Imihlathi rhoqo:
Ubomi bekholomu buxhomekeke kwizinto ezifana nokucoceka kwesigaba esihambayo, iimeko zokunyibilikisa, kunye nococeko lwezixhobo. Ukutshintshwa rhoqo kuqinisekisa iziphumo ezihambelanayo.
5.Flush iTyuwa-IziGaba eziHambayo:
Sebenzisa i-10% yesisombululo sesigaba se-organic (umzekelo, i-methanol okanye i-acetonitrile) ngaphambi nangemva kokuqhuba izigaba ze-mobile eziqulethe ityuwa ukukhusela ukuvala.
6.Gcina ngokufanelekileyo ngexesha lokuphumla:
Ukugcinwa kwexesha elide, gcina ikholamu kwi-70% yesisombululo se-organic aqueous (i-methanol okanye i-acetonitrile). Gubha nge-100% acetonitrile ngaphambi kokuba uyisebenzise kwakhona ukubuyisela ukusebenza.
7.Beka iliso kwiNtsebenzo:
Buyisela ikholamu ukuba isiphumo sayo sokubamba siyancipha okanye ukuba iimfuno zohlalutyo zigqithise amandla ayo.
Kutheni iiKholamu zeGhost-Sniper zibalulekile kwiLab yakho
IKholamu ye-Ghost-Sniper ingaphezulu kwesixhobo sokusombulula ingxaki; iphucula ukusebenza ngokubanzi kwenkqubo yakho yechromatographic.
•Susa iiNcopho zesiporho:Nakwezona meko zinzima, le kholamu ibamba iincopho zesiporho ngokufanelekileyo.
•Ikhusela izixhobo:Ihluza amasuntswana aqinileyo kunye nezingcolisi eziphilayo, ikhusela izixhobo zechromatographic kunye neekholamu.
•Ukuphucula uMgangatho weDatha:Ngokuphelisa ukuphazamiseka, ikholamu ivelisa i-chromatogram ecocekileyo, ethembekileyo.
Izixhobo zobuNzululwazi eziMaxi: Iqabane lakho kuHlahlo oluGqwesileyo
At Izixhobo zeNzululwazi zeMaxi (Suzhou) Co., Ltd., sibonelela ngezisombululo ezintsha zokuxhobisa iilabhoratri kwihlabathi liphela. IiKholamu zethu zoMoya-Sniper ziyilwe ngobuchule ukujongana nemiceli mngeni yencopho yesiporho, ukuqinisekisa iziphumo ezichanekileyo kunye nokuhamba kakuhle komsebenzi.
Phucula iChromatography yakho namhlanje
Musa ukuvumela iincopho zesiporho ziphazamise uphando lwakho. Tyala imali kwiiKholamu ze-Ghost-Sniper zeChromasir kwaye ube namava umahluko wokuchaneka, ukusebenza kakuhle, kunye noxolo lwengqondo. QhagamshelanaIzixhobo zeNzululwazi zeMaxi (Suzhou) Co., Ltd.namhlanje ukufunda ngakumbi malunga nokuba izisombululo zethu zinokuwuphakamisa njani umsebenzi welabhoratri yakho. Sikunye, masiphumelele ukugqwesa kuyo yonke ichromatogram.
Ixesha lokuposa: Dec-13-2024