Indlela yokwandisa uBomi beKholamu yakho yeChromatography

Ukugcina ikholamu yakho yechromatography ikwimeko efanelekileyo ayisiyonto nje ilungileyo yokwenza-ibalulekile kwiziphumo ezichanekileyo kunye nokusebenza kakuhle kweendleko zexesha elide. Nokuba usebenza kuhlalutyo lwamayeza, ukhuseleko lokutya, okanye uvavanyo lwendalo esingqongileyo, ukufunda indlela yokwandisa ubomi bekholamu yakho yechromatography kuya kunciphisa ixesha lokuphumla, kuphuculwe ukuphinda kuvele, kwaye kuncede ukugcina ukusebenza okungaguqukiyo.

Ukugcinwa Okufanelekileyo Kwenza Wonke Umahluko

Enye yezona zinto zingahoywayo zokugcinwa kwekholomu kukugcinwa okufanelekileyo. Iimeko ezingafanelekanga zokugcina zingakhokelela ekukhuleni kwe-microbial, ukuqhuma kwe-solvent, kunye nomonakalo ongenakuguqulwa. Ngalo lonke ixesha ulandela izikhokelo ezifanelekileyo zokugcina ezisekelwe kuhlobo lwekholamu yechromatography oyisebenzisayo. Umzekelo, xa ugcina iikholamu zesigaba esibuyiselwe umva ixesha elongeziweyo, gubha ngomxube oqulethe ubuncinci be-50% ye-solvent ye-organic, kwaye utywine zombini iziphelo ngokuqinileyo. Ukuba usebenzisa izigaba ezihambahambayo ezithintelweyo, kunqanda ukuvumela isithinteli some ngaphakathi kwikholamu, njengoko oku kunokubangela ukuna kwetyuwa kunye nokuvalwa.

Ukuthintela ukuVimba kunye nokuNgcola

Ukuphepha ukungcoliseka yenye yezona ndlela zisebenzayo zokwandisa ubomi bekholomu. Ukuhluzwa kwezigaba ezihambahambayo kunye neesampuli kubalulekile. Sebenzisa i-0.22 µm okanye i-0.45 µm yokucoca ukususa amasuntswana phambi kokutofa. Ukongeza, ukutshintshwa rhoqo kwezitywina ezigugileyo, iisirinji, kunye neesampulu zesampulu ziqinisekisa ukuba akukho nto yangaphandle ingena kwinkqubo. Kwiilabhoratri eziqhuba iimatriki eziyinkimbinkimbi okanye ezingcolileyo, ikholomu yokulinda inokusebenza njengomgca wokuqala wokukhusela kwi-sample-related fouling-absorbing contaminants ngaphambi kokuba ifike kwikholamu yohlalutyo.

Ukugungxulwa rhoqo kunye nokuCoca akuxoxiswana

Ukuba ikholamu yakho yechromatography isetyenziswa rhoqo, ukugungxulwa rhoqo kubalulekile. Ukucoca ngamaxesha athile kususa iikhompawundi ezishiyekileyo ezinokubangela ingxolo esisiseko, iincopho zesiporho, okanye ukulahleka kwesisombululo. Gubha ikholamu nge-solvent ehambelana nesigaba esihambayo kodwa yomelele ngokwaneleyo ukuhlamba nayiphi na into egciniweyo. Kwiikholamu zesigaba esibuyiselweyo, umxube wamanzi, i-methanol, okanye i-acetonitrile isebenza kakuhle. Faka ishedyuli yokucoca ngeveki esekelwe kubuninzi kunye nohlobo lohlalutyo olwenziweyo ukukhusela ukwakhiwa kunye nokuqinisekisa ukusebenza kakuhle okuphezulu.

Sebenzisa izihluzi zangaphambili kunye neeKholam zooGada

Ukufakela isihluzi sangaphambili okanye ikholamu yomlindi kutyalo-mali oluncinci olunembuyekezo enkulu. La macandelo athatha iikhompawundi kunye neekhompawundi ezigcinwe ngamandla ngaphambi kokuba zingene kwikholamu yohlalutyo oluphambili. Abagcini nje ukwandisa ubomi bekholamu yakho yechromatography kodwa bayayikhusela kwi-spikes yoxinzelelo ngequbuliso ebangelwa yimiqobo. Ngelixa ezi zincedisi zifuna ukutshintshwa ngamaxesha athile, zifikeleleka kakhulu kunokutshintsha ikholamu epheleleyo yohlalutyo.

Iingcebiso zoLondolozo lwabasebenzisi be-HPLC

Kubasebenzisi be-HPLC, ukuqwalaselwa koxinzelelo lwenkqubo kunye namazinga okuhamba kunokubonelela ngeempawu zokuqala zokuthotywa kwekholomu. Ukonyuka ngesiquphe kuxinzelelo lwangasemva ngokuqhelekileyo kubonisa ukuvaleka, ngelixa ukugcinwa kwexesha lokukhukuliseka kunokubonisa ukuvaleka okuncinci okanye ukuthotywa kwesigaba. Ukusebenzisa amanqanaba okuhamba okufanelekileyo kunye nokuphepha ukutshintshwa koxinzelelo olunobundlobongela kuya kukhusela ingqibelelo yazo zombini ukupakishwa kwekholomu kunye nesigaba sayo sokumisa. Ngaphezu koko, gwema ukuveza ikholamu kwii-solvents ezingahambelaniyo okanye iimeko ze-pH ngaphandle koluhlu olucetyiswayo, njengoko oku kunokubangela ukuwohloka ngokukhawuleza.

Iingcamango Zokugqibela

Ikholomu yakho yechromatography yinxalenye ebalulekileyo yenkqubo yakho yohlalutyo, kwaye ngononophelo olufanelekileyo, inokuhambisa amawaka eenaliti ezikumgangatho ophezulu. Ukusuka kugcino olululo ukuya ekucocweni okukhawulezileyo kunye nokuhluzwa, ukwamkela ukugcinwa kwengqondo-yokuqala akugcini nje ukugcina umgangatho wedatha yakho kodwa kunciphisa iindleko zokutshintsha.

Ngaba ujonge ukwenza ngcono ukuhamba kwechromatography kwilabhu yakho? Fumana izisombululo ezithembekileyo kunye nesikhokelo seengcali kwiChromasir-apho ukuchaneka kuhambelana nokuthembeka. Masincede sandise ubomi besixhobo sakho kwaye siphakamise iziphumo zakho.