Indlela yokuphucula uhlalutyo lwe-HLPC kunye nokuphucula ukusebenza kwelebhu

Kwiilebhu zahlalutya,I-chromatography ephezulu ye-chromatography (i-HPPC)Ngaba ubuchwephesha obufanelekileyo bokwahlula, ukuchonga, kunye nokudibanisa. Nangona kunjalo, ukufezekisa iziphumo ezingaguqukiyo nezinokuthenjwa zifuna ngaphezulu kwezixhobo ezifanelekileyo - zifunaulungiselelo. Eli nqaku libonisa indlela onokuthi uphucule ngayoUhlalutyo lwe-HPPCUkwandisa ukusebenza kakuhle, ukunciphisa ixesha lokuphumla, kunye nokuphucula ukuchaneka.

Imiceli mngeni eqhelekileyo kuhlalutyo lwe-HPPC kunye nendlela yokuzicombulula

Ngelixa i-HPLC sisixhobo esinamandla sohlalutyo, ayikho ngaphandle kwemiceli mngeni. Imiba enjeIsisombululo esingalunganga, ingxolo yesiseko, kunye neziphumo ezingahambelaniyoinokuthintela ukusebenza kwelebhu. Nantsi indlela yokulungisa ezi ngxaki ziqhelekileyo:

1. Ukusonjululwa okungalunganga

Enye yezona ngxaki ziqhelekileyo kwi-HPPC kukwahlulahlula phakathi kwencopho, rhoqo ngenxaUkukhetha okungalunganga kwekholamu okanye amaxabiso okuhamba kwe-suboptimal. Ukuphucula isisombululo:

• Khetha AIkholamu yeChromatographicNgokufanelekileyoIsigaba seSigaba soMgangathoUhlalutyi bakho.

• lungisaIxabiso lokuhamba kunye neemeko ze-gradientukuphucula i-Peak ubuqaqawuli kunye nokwahlukana.

• sebenzisaUlawulo lobushushuukuzinzisa amaxesha okugcina kunye nokuphucula ukuzala kwakhona.

I-2. Isiseko sefreyiti okanye ingxolo

Ingxolo yesiseko inokuphazamisa ukuhlolwa kwe-Peak kunye nokuPhononongwa kweDatha. Lo mbandela udla ngokubangelwa:

•Ukuguquguquka kobushushuGcina ibala lelebhu yezinzile kwaye usebenzise ikholamu yeholethi ukuba kukho imfuneko.

•Inqanaba leSoftware elingcolisekileyo-Sebenzisa izinyibilikisi eziphezulu kwaye ugcwalise isigaba sakho sefowuni ngaphambi kokusetyenziswa.

•Ungcoliseko lwesixhobo-Ukucoca rhoqo kunye nokugcina umcuphi, impompo, kunye netyhubhu yokunciphisa ingxolo yasemva.

I-3. Ukudityaniswa kwe-Peak

Ukudityaniswa okungahambelaniyo kuchaphazela ukuthembeka kokuxabatha. Ukusombulula oku:

Qinisekisa ukubaIkholamu ye-HPLC ihambelana ngokufanelekileyongaphambi kokusetyenziswa.

• Gcina AIxabiso lokuhamba elizinzileyokunye nokuthintela ukutshintsha koxinzelelo.

• YenzaUseto lwesoftware yokudityaniswa kwe-peak, qinisekisa ukuba iziphumo eziguqukayo nezibuyisiweyo.

Ukukhetha ikholamu ye-hplc efanelekileyo

Ukukhetha ikholamu ye-HPLC efanelekileyokubalulekile ukuba uphumelele ukwahlukana. Khawuqwalasele ezi zinto xa ukhetha ikholamu:

•Ubude bekholamu: Iikholamu ezinde zibonelela ngokwahlulahlula kodwa yonyusa ixesha lokuhlaziya. Khetha ubude obucombululo kunye nesantya.

•Ububanzi bekholamu: Iikholamu ezincinci zibonelela ngesisombululo esiphezulu kodwa sifuna uxinzelelo ngakumbi. Ukuqinisekiswa kwenkqubo yakho ye-HPPC.

•Isigaba sokuma: Khetha isigaba esinemichiza efanelekileyo yohlalutyi (umzekelo, i-C18 ye-onrounds, i-phenyl ye-armatic complets).

Ukwenza izigaba zesoftware kunye neereyithi zokuhamba

Inqanaba leselula liphambili kuhlalutyo lwe-HLPC. Nantsi indlela yokwandisa ngayo:

•Lungisa ukwakhiwa kwesiseko: IlungileUmyinge weSolventukuphucula ukwahlukana. SebenzisaI-LOREDIRENTkwiisampulu ezintsonkothileyo.

•Lawula amanqanaba e-PH: Qinisekisa iInqanaba leselula PHiyahambelana nesampulu kunye nekholamu.

•Yenza iqondo lokuhamba: Ireyithi yokuhamba okuphezulu inciphisa ixesha lokuhlalutyo kodwa inokubeka isuti. Isantya sengozi kunye nokusebenza kakuhle ngokusekwe kwindlela yakho.

Ulungiso kunye nokukhathalela

Isondlo esifanelekileyo sokuqinisekisaUkusebenza okungaguqukiyo kunye nokufakelwa kwe-Lifepan. Landela ezi ndlela zilungileyo:

•Ukucoca rhoqo: Hlamba rhoqoI-Inactor, ikholamu, kunye ne-cectorUkuthintela ungcoliseko.

•Faka endaweni yoko: UTSHINTSHOAmatywina, iifilitha, kunye netyhubhuNjengoko kufuneka ukunqanda ukuvuza kunye noxinzelelo lokugcwala.

•Bhala inkqubo: Rhoqo rhoqo

Ukuqukumbela

Ukuphucula uhlalutyo lwe-HPPC kubalulekile ekuphuculeni ukusebenza kwelebhu nokuqinisekisa iziphumo ezisemgangathweni eziphezulu. Ngokulungisa imiba eqhelekileyo enje ngeIsisombululo esingalunganga, ingxolo yesiseko, kunye nokudityaniswa kwe-peak yokudibanisa okungahambelaniyo, kwaye ngokukhetha ilungeloIikholamu kunye nezigaba zesoftware, ungaphucula kakhulu intsebenzo yakho yohlalutyo. RhoqoUlondolozo kunye nendlela yokusebenza ngononopheloUya kuyigcina inkqubo yakho ye-HPPC ebaleka ekusebenzeni kakuhle kwe-Deak, ukunciphisa ixesha lokuphumla kunye nokuqinisekisa iziphumo ezichanekileyo, ezichasayo.

Isikhokelo sengcali kwiUlungiselelo lwe-HPPC, nxibelelanaI-Chrosir-Khethekileyo ekuboneleleniIsisombululo se-chromatography esifihliweyoUkunceda ilabhoratri yakho ifezekise imigangatho ephezulu yokusebenza.