Kwi-High-Performance Liquid Chromatography (HPLC), ukuchaneka kunye nokusebenza kakuhle kubalulekile ekufumaneni iziphumo ezichanekileyo. Elinye lamacandelo abalulekileyo ekuqinisekiseni ukusebenza ngokufanelekileyo kwenkqubo ye-HPLC yikhangela ivalve. Nangona ihlala ingahoywa, ivalve yokutshekisha idlala indima ebalulekileyo ekulawuleni ukuhamba kwesigaba esihambayo, ukugcina ingqibelelo yenkqubo, kunye nokukhusela izixhobo ezibuthathaka njengempompo. Kweli nqaku, siza kuphonononga ukubaluleka kweevalvu zokutshekisha kwiinkqubo ze-HPLC, iintlobo zazo, imisebenzi, kunye nokubaluleka kogcino olufanelekileyo.
Indima ebalulekileyo yeeVave zokuHlola kwi-HPLC
I-valve yokutshekisha kwi-HPLC ikhusela umva ongafunekiyo we-solvents okanye izigaba ezihambahambayo kwinkqubo, iqinisekisa ukuhamba okuhambelanayo kunye nolwalathiso. Eli candelo lilula kodwa elibalulekileyo libalulekile ekuqinisekiseni iziphumo ezichanekileyo, eziphinda ziphindaphindeke zechromatographic. Nalu ujongo olusondeleyo kwimisebenzi ephambili yevalve yokukhangela:
1. Ukuthintela ukubuyela ngasemva
Umsebenzi oyintloko we-valve yokutshekisha kukuthintela umva wesigaba esihambayo okanye i-solvent. Kwiinkqubo ze-HPLC, ukugcina isikhokelo sokuhamba rhoqo kubalulekile ukuphepha ukungcoliseka okanye iziphumo ezingachanekanga. Ngaphandle kwe-valve yokutshekisha, kunokubakho umngcipheko wokubuyela umva, okunokubangela ukuxuba i-solvents, ukungcoliswa kweesampuli, okanye ukuhlukana okungafanelekanga kwee-compounds.
2. Ukukhusela impompo
Ipompo ye-HPLC yinxalenye ebalulekileyo yenkqubo eqinisekisa ukuba isigaba esihambayo sihamba ngekholomu kuxinzelelo olufunekayo. Nangona kunjalo, xa umpompo umisiwe, uxinzelelo lunokuhla, lubangele ukubuyisela umva. I-valve yokukhangela iqinisekisa ukuba uxinzelelo lugcinwa nangona ipompo ingasebenzi ngokusebenzayo, ukukhusela umonakalo kwimpompo okanye ukulahleka koxinzelelo.
3. Ukugcina Isidima seNkqubo
Iinkqubo ze-HPLC zixhomekeke kwibhalansi ebuthathaka phakathi koxinzelelo, izinga lokuhamba, kunye nokwakhiwa kwe-solvent. Ukuba ulwalathiso lokuhamba luphazamiseka ngenxa yokubuyela umva, lunokuphazamisa inkqubo yonke. I-valve yokutshekisha igcina ingqibelelo yenkqubo ngokuqinisekisa ukuba isigaba esihambayo sihamba kuphela kwicala elifunwayo, ukuphucula ukuchaneka kunye nokuhambelana kokuhlalutya.
Iindidi zokuHlola iiVave ezisetyenziswa kwi-HPLC
Iindidi ezahlukeneyo zeevalve zokutshekisha zisetyenziswa kwiinkqubo ze-HPLC, nganye eyenzelwe ukuhambelana neemfuno ezithile zokusebenza. Nazi ezinye zeentlobo eziqhelekileyo:
1. I-Spring-loaded Check Valve
I-valve yokuhlola i-spring-loaded ye-spring iyona nto isetyenziswa kakhulu kwiinkqubo ze-HPLC. Isebenzisa i-spring mechanism ukuvala ivalve xa kungekho flow okanye xa i-flow direction ijika. Olu hlobo lwevalve yokukhangela luthembekile kwaye kulula ukuyigcina.
2. Ibhola Hlola iValve
Kolu yilo, ibhola ityhalelwa kwisitulo ukunqanda ukuphuma ngasemva. Xa ukuqukuqela kuyeka, ibhola itywina ivalve, ivalela nakuphi na ukuqukuqela okubuyela umva. Iivalvu zokujonga ibhola zilula kwaye ziyasebenza, nto leyo ezenza zibe lukhetho oludumileyo kwiinkqubo ezincinci zeHPLC.
3. IValufa yokuHlola iDiphragm
Ivalve yokujonga i-diaphragm isebenzisa i-diaphragm eguquguqukayo ukutywina ivalve xa kungekho manzi aphumayo. Olu hlobo lwevalve lufanelekile kwiinkqubo ezifuna i-low-pressure, i-leak-proof seal, njengoko i-diaphragm ingakwazi ukuguquguquka ukulungiselela utshintsho oluncinci kuxinzelelo.
Ziphi iiValuvu zokuHlola ezifumaneka kwiiNkqubo zeHPLC?
Iivalvu zokujonga zihlala zibekwe kwiindawo ezicwangcisiweyo ngaphakathi kwenkqubo ye-HPLC ukuthintela ukubuya umva kwiindawo ezibalulekileyo. Ezi ndawo zinokubandakanya:
•Kwintloko yempompo:Iivalve zokujonga zivame ukufunyanwa kwindibano yempompo ukukhusela ukuhamba kwe-reverse ye-solvent kunye nokugcina uxinzelelo oluhambelanayo ngaphakathi kwenkqubo.
•Kwi-injector:Kwezinye iisistim, i-valve yokukhangela ibekwe kwi-injector ukukhusela umva ngexesha lokutofa kwesampuli, ukuqinisekisa ukuba isampuli ifakwe ngokuchanekileyo kwinkqubo.
Ukubaluleka kokuGcinwa kweValve yokuHlola
Njengawo onke amacandelo akwinkqubo ye-HPLC, iivalvu zokutshekisha zifuna ukugcinwa rhoqo ukuqinisekisa ukuba zisebenza ngokugqibeleleyo. Ngokuhamba kwexesha, iivalve zokukhangela zinokuvaleka ngamacandelo, zonakaliswe zizinyibilikisi, okanye amava anxibe kwaye akrazuke ngenxa yokusetyenziswa ngokuphindaphindiweyo. Oku kunokukhokelela kwimiba enjengokuvuza, ukulahleka koxinzelelo, okanye ukuhamba okungahambelaniyo. Ukuhlolwa rhoqo, ukucocwa, kunye nokutshintshwa kweevalvu zokutshekisha kunokuthintela le miba, ukuqinisekisa ubomi obude benkqubo yakho ye-HPLC kunye nokugcina umgangatho weziphumo zakho.
Isishwankathelo, i-valve yokutshekisha kwinkqubo ye-HPLC idlala indima ebalulekileyo ekugcineni ukuhamba okufanelekileyo kwesigaba esihambayo, ukukhusela umva, kunye nokukhusela amacandelo abalulekileyo njengempompo. Ngokuqonda umsebenzi wayo kunye nokugcina eli candelo lilula kodwa liyimfuneko, unokuphucula ukuchaneka, ukusebenza kakuhle, kunye nobomi obude benkqubo yakho ye-HPLC. Nokuba wenza uhlalutyo lwesiqhelo okanye usebenza kwimisebenzi entsonkothileyo yechromatographic, ungakuhoyi ukubaluleka kwevalve esebenza ngokufanelekileyo ekuqinisekiseni ukusebenza kakuhle kwenkqubo.
Ukugcinwa rhoqo kunye nokuqonda iintlobo zeevalvu zokukhangela ezikhoyo kunokunceda ukunciphisa imiba kunye nokuphucula ukuthembeka kwenkqubo yakho ye-HPLC.
Ixesha lokuposa: Nov-07-2024