Kutheni i-HPPC ibalulekile kwi-labs yophando

Kwi-chromatography ye-chromatography ye-chromatography ephezulu (i-HPPC), yonke into idlala indima ebalulekileyo ekufezekiseni iziphumo ezichanekileyo nezinokuthenjwa. Phakathi kwezi macandelo, ityhubhu ye-HPPC inokubonakala ikwinqanaba lesibini, kodwa ibaluleke kakhulu ekuqinisekiseni ukungqinelana nokuchaneka kwe-LOBS. Ukuqonda ukuba kutheni i-HPPC ibalulekile kwaye indlela yokukhetha ilungelo unokwenza wonke umahluko kumgangatho weziphumo zakho zelebhu.

Indima ye-HPPC ye-tubc kwi-labs yophando

I-HPPC ye-tubing izenzoNjengendlela yesampulu yereyitha kunye nesinyibilikisi yokuhamba kwinkqubo ye-HPPC. Kwanangahluka okuncinci kwityhubhu kunokuba nefuthe amaxabiso okuhamba, uxinzelelo, kunye nomgangatho wokwahlukanisa. Abaphandi bajolise kwiziphumo zokuzala, ukukhetha ityhubhu efanelekileyo. Ngezicelo kwiindawo zamachiza, uhlalutyo lokusingqongileyo, kunye ne-bichemistry, ukhetho lwe-HPLC tub ifuthe ngokuthe ngqo ukuchaneka koluhlu olubanzi lwezifundo.

1. Imicimbi ebonakalayo: Ukukhetha ityhubhu elungileyo

Izinto ezibonakalayo ze-HPPC zichaphazela kakhulu ukusebenza. Intsimbi engenasitali, i-Peek (i-Polyteether Ether Itone), kwaye i-Ficea i-Ficea zizixhobo eziqhelekileyo, nganye ilungele iintlobo ezithile zohlalutyo. Umzekelo, ityhubhu yentsimbi engenasitali iqine kwaye ixhathise uxinzelelo oluphezulu, lwenza ukuba lilungele uvavanyo oluphezulu. I-Peek, kwelinye icala, i-Inher engaphandle kunye ne-non-metric, iyenza ilungele izicelo ze-biologiz apho i-ion yentsimbi inokuphazamisa i-itrand ethobekileyo.

Isifundo seNqanaba leStails vs

Kwisifundo kwii-submations zamayeza, ilebhu ifumanise ukuba ityhubhu yentsimbi engenasitayile inike iziqhamo eziphezulu kodwa zichaphazele kancinci kancinci. Ukutshintshela kwi-Peek Tubing ekupheliseni lo mbandela, ukubonisa ukubaluleka kokhetho lwezinto ezibonakalayo ekugcineni ingqibelelo yesampulu.

2. Ububanzi bayo kunye nefuthe layo ekuhambeni

Ububanzi be-HPPC ye-HPPC yenye into ebalulekileyo. Ububanzi obuncinci bangaphakathi banokunceda ekufezekiseni ubuntununtunu obukhulu ngokunciphisa i-band ukundiswa, kodwa kukwafuna ulawulo lonoxinzelelo olufanelekileyo. Kwelinye icala, ububanzi obukhulu buhlala bulungele amaxabiso okuqala ngokukhawuleza kodwa unokunciphisa isigqibo. Ukukhetha ityhubhu kunye ne-diameter efanelekileyo kubalulekile ukulinganisa ubuntununtunu ngexabiso lokuhamba kunye neemfuno zoxinzelelo.

Yenza i-tubing ye-tubing yohlalutyo okanye i-hplc yokulungisa i-hplc

Ukuhlalutya i-HPPC, idayimitha encinci yangaphakathi (umzekelo, 0.13 mm) ihlala ibonelela ngokwahlula-hlulwa ngcono. Ngokwahlukileyo, i-HPLC yokulungiselela, ehambisa imiqulu emikhulu yesampulu, ihlala izibonelelo kumzobo omkhulu wokuxhasa ukuhamba okukhawulezileyo kunye nokunciphisa umva.

I-3. Ubude kunye noxinzelelo: Ukufumana ibhalansi efanelekileyo

Ubude be-HPPC buchaphazela zombini indlela yokuhamba kunye nengcinezelo yenkqubo. Ukuthetha ixesha elide kunokukhokelela kuxinzelelo olongezelelekileyo, olunokuthi lufune uhlengahlengiso kwi-Pump. Oku kubaluleke kakhulu kwizicelo zoxinzelelo oluphezulu njenge-hplc ye-hplc, apho ubude betyhefu buchaphazela ngokuthe ngqo ixesha lokugcina kunye nomgangatho wokwahlukana. Ukugcina ityhubhu njengokufutshane kangangoko kunokwenzeka ngaphandle kokuthintela amanqaku onxibelelwano unokunceda ekufezekiseni ibhalansi efanelekileyo yoxinzelelo.

I-tubfien tub yokunciphisa uxinzelelo lwenkqubo

Kwizicelo zoxinzelelo oluphezulu, ukunciphisa ubude betyhefu kunokunciphisa ukwakha koxinzelelo, ukuphucula uzinzo lwenkqubo kunye nokugcina impompo. Iilebhu eziqhuba uhlalutyo lwegalelo eliphezulu zixele ukwehla okubonakalayo kwiimfuno zesondlo ngokulandelelana kobude bokwenyani.

4. Ukuhambelana neekhemikhali kunye nezinyibilikisi

Ukungqinelana kwe-HPPC yetyhubhu enemichiza eyahlukeneyo kunye nezinyibilikisi kubalulekile, ngakumbi kwiilebhu eziphatha uluhlu olubanzi lwezinto ezihlanganisiweyo. Izinyibilikisi ezithile zinokuthambisa i-Teubing Stop Onfing ngokuhamba kwexesha, ezikhokelela kungcoliseko okanye ukuvuza. Ngaphambi kokukhetha ityhudiyo, qinisekisa ukuba i-faces yayo isetyenziswa rhoqo kwilebhu yakho ukuthintela le micimbi.

UMZEKELISO WOKUGQIBELA WOKUGQIBELA

Ilebhu yovavanyo lokusingqongileyo iqhuba uhlalutyo lwezinambuzane ezifumanise ukuba izinto zayo zetyhubhu zazingahambelani nezisombululo ezithile ezisetyenziswayo kuvavanyo, zibangelwa kukungafakwa endaweni. Ukutshintshela kwi-sub ehambelana ne-cheming ehambelana noko kulungiswa kwaye kuphuculwe ukuthembeka kwesiphumo.

5. Ukuqinisekisa ukuba ucocekile kwaye ungcoliseko olungathethekiyo

Ungcoliseko lunokulalanisa ngokulula iziphumo ze-HLLC, kwaye ityhubhu inokuba ngumthombo ofihlakeleyo walo mbandela. Ukucocwa kwesiqhelo kunye nokutshintshwa kokuqhelekileyo kwetyhubhu kunceda ukugcina ingqibelelo yenkqubo ye-HPPC. Iilebhu ezininzi zibandakanya ulondolozo olucwangcisiweyo kwaye zithathe indawo yokuthintela amaxesha ngamaxesha ukunqanda umngcipheko wokungcola, ngakumbi kwizixhobo eziphezulu ezifana nophando lwamayeza kunye ne-biochemical.

Seka indlela yokugcina i-tyunca

Ukuqokelela uhlolo rhoqo kunye neeprothokholi zokucoca i-HPPC Tubing kungathintela ukwakha i-Supersue kunye nongcoliseko, olukhokelela kwiziphumo ezingaphezulu. Ezinye iilebhu zisebenzisa ukucheba okanye imijikelezo echongiweyo yokuqinisekisa ukuba ityhubhu ingena kwintsalela.

Ukhetho olufanelekileyo lwe-HPPC lunokuphucula kakhulu ukusebenza nokuchaneka kweelebhu zophando. Ukusuka ekukhetheni izinto ezifanelekileyo kunye nobubanzi boxinzelelo kunye nokuqinisekisa ukuhambelana nemichiza, ukuqwalaselwa ngasinye kunempembelelo ukusebenza ngempumelelo kohlalutyo lwe-HPPC. Ngokunika ingqalelo ngenyameko ezi zinto, abaphandi banokufumana iziphumo ezinokuthenjwa, eziphikisayo eziqhubela phambili izifundo zabo kwaye zinegalelo kwinkqubela phambili ebanzi yesayensi. Ukugcinwa kwetyholo elifanelekileyo kunye nokukhethwa kungekuphela nje i-lab isebenza ngokuphumelelayo kodwa kukhusela umgangatho weziphumo zophando, ukwenza i-HPPC ye-tubc tytor enezinto ezisisiseko.